Answer:
Option 2, thumb and forefinger
Explanation:
When putting on the first glove in performing sterile gloving, the nurse will want to use the thumb and forefinger (or index finger) of their non-dominant hand to grasp the edge of this glove's folded cuff. By grabbing the edge of this glove with the lowest amount of fingers necessary, the nurse will be touching a portion of glove that is closer to its inside surface (the part that will be touching the nurse's skin regardless) while minimizing the amount of contact potential contact that can be made between the clean hand and the sterile surfaces of the glove that must remain sterile. If the clean hand touches the sterile surface, the glove is now contaminated and the process must restart.
The nurse will then place their firm dominant hand, with the palmar surface facing downward, into the glove. If the fingers do not insert into their proper placements, the nurse should wait until they don the second glove on their nondominant hand before fixing them.
Donning the second glove is where the nurse will grasp the folded cuff with the 2nd, 3rd, and 4th fingers, opening it for the nondominant hand to insert. The nurse can do this with the second glove because the dominant hand is now sterile and can make more contact with the contents of the sterile field than before.
In short, the nurse must use the thumb and forefinger to grasp the sterile glove's folded cuff, option 2.