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what would you expect to see if you plated some of your transformed bacteria onto a plate without antibiotic? would there be an advantage to doing this (in terms of understanding your results)? explain.

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We would observe the emergence of new types of contaminating microorganisms.

This would be beneficial since it would show how the changed bacteria would interact with the bacteria that are naturally present around them.

Bacteria may be able to quickly adapt to their surroundings by modifying their environmental DNA. This may help bacteria by promoting the spread of antibiotic resistance, but it may also be detrimental if it eliminates advantageous genes from a population.

Sanger sequencing is the most accurate method of confirming your recombinant colonies. The first step is to separate the plasmid DNA from an overnight bacterial growth.

When everything is finished, the insert can be located using sequencing primers designed for the chosen vector.

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