The chemical energy generated by oxidation reactions cannot be captured by NADH.
The pyruvates produced during glycolysis are then converted into lactic acid via oxidation of NADH.
The molecule exists in two states: oxidized (NAD+) and reduced (NADH), depending on how many electrons and protons it has taken which together will be the equivalent of a hydrogen atom along with an extra electron.
The mitochondrion receives pyruvate, the byproduct of glycolysis, and transforms it into acetyl coenzyme A, also known as acetyl CoA. The transfer of electrons from pyruvate to acetyl CoA also causes the formation of NADH, which is a kind of energy storage.
Therefore, the chemical energy generated by oxidation reactions cannot be captured by NADH.
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