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Concentration of bacteria per milliliter of beef is 6.7 × 103 CFU/ml .
10-6 dilution can be targeted by using three 1:100 dilutions, or six 1:10 dilutions, or a combination of 100-fold and 10-fold dilutions. It must be brought to mind that in order to achieve a 10-6 dilution, 1.0 ml of sample from the last dilution must be plated.
10-2 dilution means a “one to one hundredth dilution”, so to make this dilution Mix 1 in 100 total or 5 in 500 total or 10 in 1000 total. A two-fold dilution reduces the concentration of a solution by a factor of two that is reduces the original concentration by one half.
A common rule for calculating the concentration of solutions in a series is to multiply the original concentration by the first dilution factor, this by the second dilution factor, this by the third dilution factor, and so on until the final concentration is reached .
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