Respuesta :
HDL has vital immunomodulatory properties, which includes the attenuation of lipopolysaccharide (LPS)-precipitated inflammatory reaction.
- As lecithin-ldl cholesterol acyltransferase (LCAT) is a important enzyme withinside the maturation of HDL we investigated whether or not LCAT-deficient (Lcat(-/-)) mice gift an improved LPS-precipitated inflammatory reaction.
- LPS (100μg/kg frame weight)-precipitated cytokine reaction in Lcat(-/-) mice become markedly improved and extended as compared to wild-kind mice. Importantly, reintroducing LCAT expression the usage of adenovirus-mediated gene switch reverted their phenotype to that of wild-kind mice. Ex vivo stimulation of complete blood with LPS (1-100ng/mL) confirmed a comparable improved pro-inflammatory phenotype.
- Further characterization in RAW 264.7 macrophages in vitro confirmed that serum and HDL, however now no longer chylomicrons, VLDL or the lipid-unfastened protein fraction of Lcat(-/-) mice, had a discounted ability to minimize the LPS-precipitated TNFα reaction.
Learn more about HDL:
https://brainly.com/question/841110
#SPJ4
