A group of researchers wanted to sort different white blood cell types (monocytes, lymphocytes, and granulocytes) apart from each other based on size differences and to remove unwanted contaminating red blood cells. After a particular manipulation, the red blood cells lysed. The remaining white blood cells increased in size and, more importantly, the size differences among cells increased, allowing for size-based sorting (which requires minimum size differences among cells). What manipulation did the researchers use to increase cell size?A. placing cells in an environment with lower temperatures than the cells were previously exposed toB. patch-clamp recording to monitor ion channel activityC. placing cells in an environment with a lower solute concentration than that in the cellsD. placing cells in an environment with a higher solute concentration than that in the cells

In pathological or diagnostic laboratories, to perform the differential leukocytes count (DLC) or white blood cells (WBC) count which are granulocytes, lymphocytes and monocytes we should first lyse the erythorcytes or red blood cells (RBC) with RBC lysis buffer. When RBC's lysis occur then only WBC's remain in the solution. Then, we add the hypotonic solution or lower solute concentration solution. By adding the hypotonic solution the cells will swell and increase in size.

So, the researchers used the hypotonic solution or lower solute concentration solution to increase the size of cells to differentiate between them.

Note:

In hypotonic solution the cells are increased in size or swell.

In hypertonic solution the cells are decrease in size or shrink.

In Isotonic solution the cells size remain same as normal.