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During the final step of cellular respiration (electron transport chain & oxidative phosphorylation), the mitochondria pumps H+ ions from the matrix (the innermost part of the mitochondria) to the intermembrane space. This creates something called a concentration gradient. After all the H+ ions are pumped into this space, where do H+ ions want to move and more importantly why?

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kayobrien2025
Overview of oxidative phosphorylation. The electron transport chain forms a proton gradient across the inner mitochondrial membrane, which drives the synthesis of ATP via chemiosmosis

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