A sample is subjected to UV-Vis spectroscopy in a 1 cm cell, and 23.2% of the incident light is transmitted through the sample to the detector. If the molar absorptivity of the analyte in the sample is 450 L/(mol*cm), what is the concentration of the analyte in the sample?

Respuesta :

Answer:

0.001410 mol/L is the concentration of the analyte in the sample.

Explanation:

Using Beer-Lambert's law :

Formula used :

[tex]A=\epsilon \times C\times l[/tex]

[tex]A=\log \frac{I_o}{I}[/tex]

[tex]\log \frac{I_o}{I}=\epsilon \times C\times l[/tex]

where,

A = absorbance of solution

C = concentration of solution = [tex]2.00\times 10^{-3}M[/tex]

l = path length = 1.00 cm

[tex]I_o[/tex] = incident light

[tex]I[/tex] = transmitted light

[tex]\epsilon[/tex] = molar absorptivity coefficient

Here we are given : [tex]\epsilon = 450 L/(mol cm)[/tex]

Transmittance of light = T = 23.2%

[tex]T=\frac{I}{I_o}\times 100[/tex]

[tex]23.2\%=\frac{I}{I_o}\times 100[/tex]

[tex]0.232=\frac{I}{I_o}[/tex]

[tex]I=0.232\times I_o[/tex]

[tex]A=\log \frac{I_o}{I}[/tex]

[tex]A=\log \frac{I_o}{0.232\times I_o}=0.6345[/tex]

[tex]A=\epsilon \times C\times l[/tex]

[tex]C=\frac{A}{\epsilon \times l}=\frac{0.6345}{450 L/(mol cm)\times 1 cm}[/tex]

C = 0.001410 mol/L

0.001410 mol/L is the concentration of the analyte in the sample.