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The locomotion of fibroblasts in culture is immediately halted by the drug cytochalasin, whereas colchicine causes fibroblasts to cease to move directionally and to begin extending lamellipodia in seemingly random directions. Injection of fibroblasts with antibodies to vimentin has no discernible effect on their migration. What do these observations suggest to you about the involvement of the three different cytoskeletal filaments in fibroblast locomotion?

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Answer:

Vimenin has no affect.

Cytochalasin interferes with actin filament formation;

Ccolchicine shows that microtubules are required to give a cell a polarity that the determines which end becomes the leading edge.

Explanation:

These observations suggest to me that

  • Vimenin has no affect.
  • Cytochalasin interferes with actin filament formation;
  • Colchicine shows that microtubules are required to give a cell a polarity that the determines which end becomes the leading edge.

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