If you mixed the mRNA of a human gene with the genomic DNA for the same gene and allowed the RNA and DNA to form a hybrid, what would you be likely to see in the electron microscope? Your figure should include hybridization involving both DNA strands (template and RNA-Iike) as well as the mRNA.

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Answer:

Explanation:

A gene in the DNA has both exons and introns. Exons are the coding sequences that will remain in the mature mRNA, and introns are non-coding sequences that are spliced out and are not a part of the mature mRNA.

If you hybridize genomic DNA with a mature mRNA, in a microscope you'll probably see something like the figure I'm attaching. In A you can see a schematic diagram of the mRNA-DNA hybrid; the loops are single stranded DNA containing introns, that are not present in the mRNA and therefore remain unpaired.

In figure B you can see a real image of a mRNA-DNA hybrid and its interpertation below.

The hybridization involving both DNA strands would look like a perfect pairing, because both DNA strands contain complementary sequences of exons and introns.

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