A plasmid vector contains a single ecori site in the polylinker (multiple cloning site). a gene of interest is flanked by two ecori sites, one at each end. you cut the vector and the dna containing your insert with ecori, and ligate the two. how many ecori site(s) is/are present in the recombinant dna molecule and how many band(s) would you see when you run your digest of recombinant dna on a gel? (assume that there is no internal ecori site in the insert dna.)

There are TWO EcoRI restriction sites after ligation, thereby I expect to see TWO bands on an electrophoresis gel after digestion.

EcoRI is a restriction endonuclease enzyme that recognizes and cuts the DNA sequence 5'-GAATTC'-3 between Guanine (G) and Adenine (A).

This recognition sequence is a palindrome, thereby the EcoRI enzyme also recognizes the 3' CTTAAG 5' at the opposite strand and again cuts between A and G.

In this case, the recombinant DNA will have

One (1) recognition sequence for the EcoRI enzyme localized in the recognition site of the plasmid vector and
One (1) recognition sequence localized in the site where the ligase enzyme produced the ligation of the resulting circular recombinant DNA molecule.

In consequence, EcoRI can cut the resultant recombinant DNA in two different restriction sites and therefore two bands can be observed in an electrophoresis gel after EcoRI digestion.

In conclusion, there are TWO EcoRI restriction sites after ligation, thereby I expect to see TWO bands on an electrophoresis gel after digestion.

Learn more in:

https://brainly.com/question/9282378?referrer=searchResults