Due to the fact that neither Hfr nor F+ cells may serve as recipients, this conjugation cannot occur. In conjugation, the recipient cells must be F-.
The state of F in a cell is described using a few unique terms: F+ denotes a strain with an F plasmid, while F- denotes a strain lacking any kind of F. F is extremely effective at moving from an F+ cell to an F- cell. About 1/10 of the F- cells will become F+ after co-culturizing with F+ cells.
F+ bacteria are cells that have the fertility factor plasmid, which enables the cells to begin conjugation. When an F+ cell and an F- cell are conjugated, the plasmid is transmitted but no bacterial genes are. F- cells are identical cells lacking this plasmid that confers fertility.
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